Typing of Mycoplasma pneumoniae by PCR-mediated DNA fingerprinting.
نویسندگان
چکیده
PCR fingerprinting was used to characterize clinical isolates of Mycoplasma pneumoniae. Among 24 strains tested, two types were distinguished. Nineteen strains belonged to type 1, whereas only 5 strains belonged to type 2. The majority of strains isolated since 1991 in Belgium belong to type 1. No variations in fingerprinting patterns were observed within each group, confirming the highly conserved nature of the M. pneumoniae genome.
منابع مشابه
DNA Fingerprinting Based on Repetitive Sequences of Iranian Indigenous Lactobacilli Species by (GTG)5- REP-PCR
Background and Objective: The use of lactobacilli as probiotics requires the application of accurate and reliable methods for the detection and identification of bacteria at the strain level. Repetitive sequence-based polymerase chain reaction (rep-PCR), a DNA fingerprinting technique, has been successfully used as a powerful molecular typing method to determine taxonomic and phylogenetic relat...
متن کاملRandom amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
متن کاملCOMPARISON OF FOUR PCR TESTS FOR THE DETECTION OF MYCOPLASMA PNEUMONIAE
Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, det...
متن کاملPrevalence of Mycoplasma pneumonia in Patients with Respiratory Infections from Shaheed Mostafa Khomeini and Khatam Hospitals by Culture and PCR Methods
Background and Aims: Mycoplasma pneumoniae is one of the most important pathogens causing human respiratory tract infection; especially in community-acquired pneumonia (responsible for 10 – 40% of these infections).The aim of this study was to evaluate the prevalence of Mycoplasma pneumonia in patients with respiratory infections from Mostafa Khomeini and Khatam hospitals, by culture and molecu...
متن کاملComparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains.
The aim of this study was to identify the strengths and weaknesses of five DNA fingerprint methods for epidemiological typing of Streptococcus pneumoniae. We investigated the usefulness of (i) ribotyping, (ii) BOX fingerprinting with the BOX repetitive sequence of S. pneumoniae as a DNA probe, (iii) PCR fingerprinting with a primer homologous to the enterobacterial repetitive intergenic consens...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 32 11 شماره
صفحات -
تاریخ انتشار 1994